Objective: To identify levels of physical activity, depression and cardiovascular risk in the teaching and other staff of a university in Medellin, Colombia (Politécnico Colombiano Jaime Isaza Cadavid). Method: A random sample was selected of teachers and other staff born since 1955, stratified by the Framingham age range. Lipid profile and glycemia levels were measured and medical and anthropometric evaluations were carried out. The International Physical Activity Questionnaire (Short-IPAQ) and the Hamilton depression scale were administered. Results: A total of 45.4% of the participants were sedentary, 40.5% had mild or moderate depression, 10.5% consumed 22 or more grams of alcohol per week, 7% were hypertensive, 75.6% had dyslipidemia, 3.5% had diabetes, 18.6% were obese, and 19.8% were smokers. Low-density lipoprotein cholesterol (LDL-C) levels were classified as borderline or high risk in 79.1% and high-density lipoprotein cholesterol (HDL-C) as borderline, high or moderate risk, respectively, in 75.6%. The total/HDL cholesterol ratio showed risk in 43% of the participants. According to the Framingham scale, 31.4% had a medium, moderate or high risk of having a heart attack within the next 10 years. Conclusions: This descriptive analysis reveals that the main cardiovascular risk factors in this population were lipid disorders and physical inactivity. Lower levels of LDL-C and depression were found in physically active or highly active participants.