Introduction: Previous studies have found that nonspecific exercises alter some hormonal concentrations. Tests able to detect adaptation specifically to judo should be designed. In an initial study, we demonstrated that a specific force-velocity test affects levels of the hormones studied, which should be included in medical monitoring. Objective: To explore the advisability of including some hormonal variables in the medical monitoring of specific resistance training in judo. Methods: A specific resistance test was applied to 12 judoists in the Cuban female national preselection (age 19.5 ± 1.8 years; weight: 69.5 ± 21.5 kg; fat: 22.4 ± 7.4%). Heart rate and blood concentrations of lactate, growth hormone (GH), cortisol, and prolactin were registered. Results: Heart rate and lactate concentrations showed highly significant differences between baseline and post-load values (196% and 187% respectively). Equally, GH (176%) and prolactin levels (95%) also showed highly significant increases. Only minor changes were observed in cortisol levels (35%). Conclusions: These results indicate that this test is able to cause a response in the hormones studied and its use is advisable to evaluate specific resistance capacity in these sportswomen. As in the force-velocity test, the response of these hormones should be determined according to training stage, weight divisions, intensity of exercise and work volume. Standard values to evaluate this hormonal response should be established.